Which ALP isoenzyme is the most heat-stable?

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Multiple Choice

Which ALP isoenzyme is the most heat-stable?

Explanation:
Heat stability of alkaline phosphatase isoenzymes is what's being assessed. Placental alkaline phosphatase is the most heat-stable form among the ALP isoenzymes. In lab practice, heating a serum sample (for example, 56°C for 10 minutes) will inactivate most ALP isoenzymes from liver, bone, and intestine, causing a large drop in activity. The placental isoenzyme, however, resists heat-induced denaturation and retains a substantial portion of activity. This difference in thermal stability comes from structural variations in PLAP, such as its subunit composition and glycosylation, which confer greater resistance to heat. As a result, after heating, the remaining ALP activity is most consistent with placental isoenzyme, making it the correct choice.

Heat stability of alkaline phosphatase isoenzymes is what's being assessed. Placental alkaline phosphatase is the most heat-stable form among the ALP isoenzymes. In lab practice, heating a serum sample (for example, 56°C for 10 minutes) will inactivate most ALP isoenzymes from liver, bone, and intestine, causing a large drop in activity. The placental isoenzyme, however, resists heat-induced denaturation and retains a substantial portion of activity. This difference in thermal stability comes from structural variations in PLAP, such as its subunit composition and glycosylation, which confer greater resistance to heat. As a result, after heating, the remaining ALP activity is most consistent with placental isoenzyme, making it the correct choice.

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